January 1, 2018

MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells

Authors:

Christine Koehler, Edward A Lemke

(*) equal first authors

doi:

10.1007/978-1-4939-7574-7_19

Abstract:

The combination of genetic code expansion (GCE) and baculovirus-based protein expression in Spodoptera frugiperda cells is a powerful tool to express multiprotein complexes with site-specifically introduced noncanonical amino acids. This protocol describes the integration of synthetase and tRNA gene indispensable for GCE into the backbone of the Bacmid, the Tn7-mediated transposition of various genes of interest, as well as the final expression of protein using the MultiBacTAG system with different noncanonical amino acids.

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